The NEXTflex™ 16S V5 – V6 Amplicon-Seq Library Prep Kit is designed for bacterial profiling of plant samples using degenerate primers that target the fifth and sixth variable regions of the 16S rRNA gene . Bacterial profiling in plant samples can be difficult because of the homology between bacterial 16S rRNA and chloroplast 16S rRNA. The use of primers binding to 16S hypervariable regions V 1 – V4, which are traditionally used for bacterial profiling, amplify both the bacterial and plant 16S rRNA genes, skewing results. NEXTflex™ 16S V5 – V6 Amplicon-Seq libraries are compatible with paired-end sequencing on the Illumina ® MiSeq ® sequencing platform and are ideal for monitoring population fluctuations of microbial communities.
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